|
| Status |
Public on Feb 23, 2023 |
| Title |
healty volunteer 5 |
| Sample type |
RNA |
| |
|
| Source name |
Plasma
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: non-spinal tuberculosis
cell type: Plasma
molecule subtype: microRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from 200 μl of plasma using a miRcute miRNA extraction kit (TIANGEN, China) according to the manufacturer’s protocol.
|
| Label |
Hy3™
|
| Label protocol |
After quality control, the miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
The Hy3™-labeled samples were hybridized on the miRCURYTM LNA Array (v.19.0) (Exiqon) according to the array manual.
|
| Scan protocol |
using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
|
| Description |
X9
After quality control, the miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s protocol. The Hy3™-labeled samples were hybridized on the miRCURYTM LNA Array (v.19.0) (Exiqon) according to the array manual. And the data were collected using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
|
| Data processing |
Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=30 in all samples were chosen for calculating normalization factor
|
| |
|
| Submission date |
Feb 20, 2023 |
| Last update date |
Feb 24, 2023 |
| Contact name |
Zili Wang |
| E-mail(s) |
zili.wang@nyfy.com.cn
|
| Organization name |
Xi’an International Medical Center Hospital Affiliated to Northwest University
|
| Department |
Spine Surgery
|
| Street address |
NO.737, Xitai Road, High-tech Zone
|
| City |
Xi’an |
| ZIP/Postal code |
710000 |
| Country |
China |
| |
|
| Platform ID |
GPL33147 |
| Series (1) |
| GSE225679 |
microRNAs expression Profiles in plasma from spinal tuberculosis patients and healthy volunteers |
|
