|
| Status |
Public on Sep 14, 2023 |
| Title |
LNCaP_EHMT1_K450R451R_DOXUNC_B_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
LNCaP-tet-EHMT1-K450/451R
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: LNCaP-tet-EHMT1-K450/451R
treatment: Treated with 0.5ug/mL doxycyline and 2uM of UNC0642
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Rneasy Mini Kit (QIAGEN) following manufacturer’s protocol.
RNA-seq libraries were performed by using TruSeq® Strnd Total RNA LT (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Description |
LNCaP_EHMT1_K450RK451R_UNC_count_table.txt.gz
|
| Data processing |
The sequence quality of raw reads was checked by FastQC (version 0.11.9).
Raw reads were aligned to the human reference genome (hg19) using STAR (version 2.7.2) followed by counting with featureCounts (version 1.6.4) from GRCh37 Ensembl reference.
Assembly: hg19
Supplementary files format and content: txt file for raw counts of sequence reads
|
| |
|
| Submission date |
Feb 10, 2023 |
| Last update date |
Sep 14, 2023 |
| Contact name |
Changmeng Cai |
| E-mail(s) |
changmeng.cai@umb.edu
|
| Organization name |
University of Massachusetts Boston
|
| Street address |
100 William T Morrissey Blvd
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02125 |
| Country |
USA |
| |
|
| Platform ID |
GPL30173 |
| Series (2) |
| GSE201771 |
Lysine methylation of EHMT1/GLP as a molecular switch to reprogram transcription networks in prostate cancer |
| GSE225068 |
Lysine methylation of EHMT1/GLP as a molecular switch to reprogram transcription networks in prostate cancer [RNA-seq UNC0642] |
|
| Relations |
| BioSample |
SAMN33244429 |
| SRA |
SRX19333431 |
