|
| Status |
Public on Sep 23, 2011 |
| Title |
p52-D-L |
| Sample type |
RNA |
| |
|
| Source name |
diagnosis
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: acute lymphoblastic leukemia (ALL)
tissue: bone marrow
relapse time: late (greater than 36 months)
|
| Treatment protocol |
Leukemic bone marrow samples were used for RNA extraction without further treatment
|
| Growth protocol |
Ficoll-enriched, cryopreserved paired diagnosis/relapse bone marrow samples were obtained from patients with relapsed B-precursor acute lymphoblastic leukemia.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen RNEasy Mini Kits (Valencia, CA) and quality was verified by an agilent 2100 Bioanalyzer (Palo Alto, CA)
|
| Label |
biotin
|
| Label protocol |
One microgram of total RNA was used as a template in a double amplification protocol using RiboAmp RNA amplification kits (Arcturus, Mountain View, CA). In vitro transcription was completed with biotinylated UTP and CTP for labeling using ENZO BioArray HighYield RNA Transcript Labeling kit (ENZO Diagnostics, Farmingdale, NJ)
|
| |
|
| Hybridization protocol |
Thirteen micrograms of labeled cRNA was fragmented and hybridized to Affymetrix U133Plus2 microarrays according to Affymetrix protocol (Santa Clara, CA).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
pediatric patients with B-precursor ALL
|
| Data processing |
The data were analyzed with GenePattern.
|
| |
|
| Submission date |
Apr 08, 2011 |
| Last update date |
Nov 14, 2018 |
| Contact name |
Laura E. Hogan |
| E-mail(s) |
laura.hogan@stonybrook.edu
|
| Phone |
631-444-7720
|
| Fax |
631-444-2785
|
| Organization name |
Stony Brook University
|
| Department |
Department of Pediatrics
|
| Street address |
HSC T11-049
|
| City |
Stony Brook |
| State/province |
New York |
| ZIP/Postal code |
11794 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE28460 |
Expression data from ALL diagnosis and relapse pediatric acute lymphoblastic leukemia cases |
| GSE28462 |
Integrated Genomic Analysis of Relapsed Childhood Acute Lymphoblastic Leukemia reveals therapeutic strategies |
|
| Relations |
| Reanalyzed by |
GSE122511 |
