|
| Status |
Public on Sep 10, 2011 |
| Title |
PS2-2 iPSC |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
undifferentiated PS2-2 iPSC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: iPSC
|
| Treatment protocol |
Cells were washed with PBS and Dneasy kit was used to isolate total DNA.
|
| Growth protocol |
Primary fibroblasts were cultured in DMEM supplemented with 10% FBS. iPSC were cultured in hES medium containing bFGF.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA samples were isolated using DNeasy kit.
|
| Label |
cy5
|
| Label protocol |
500 ng of DNA was labelled using the Enzo Genomic DNA Labelling kit.
|
| |
|
| Channel 2 |
| Source name |
Human Genomic DNA
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Promega, female(G1521)
|
| Treatment protocol |
Cells were washed with PBS and Dneasy kit was used to isolate total DNA.
|
| Growth protocol |
Primary fibroblasts were cultured in DMEM supplemented with 10% FBS. iPSC were cultured in hES medium containing bFGF.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA samples were isolated using DNeasy kit.
|
| Label |
cy3
|
| Label protocol |
500 ng of DNA was labelled using the Enzo Genomic DNA Labelling kit.
|
| |
|
| |
| Hybridization protocol |
Hybridizations were performed on slides containing four arrays, with each array containing 622060 in-situ synthesized 60-mer oligonucleotides, representing 170344 unique chromosomal locations (Agilent Technologies).
|
| Scan protocol |
Images of the arrays were acquired using a microarray scanner G2505CA (Agilent technologies) and image analysis was performed using feature extraction software version 10.7 (Agilent Technologies).
|
| Data processing |
The date were imported into Agilent Genomic Workbench using the aberration detection method 2 (ADM-2) algorithm (10.0 threshold) for further analysis.
|
| |
|
| Submission date |
Apr 07, 2011 |
| Last update date |
Sep 10, 2011 |
| Contact name |
Takuya Yagi |
| E-mail(s) |
takuyagi@2006.jukuin.keio.ac.jp
|
| Organization name |
Keio University
|
| Street address |
35-Shinanomachi Shinjuku-ku
|
| City |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10123 |
| Series (1) |
| GSE28450 |
aCGH analysis of familial Alzheimer’s disease with presenilin 2 mutation patient-specific induced pluripotent stem cells |
|
