|
Status |
Public on May 02, 2012 |
Title |
0.1%_DMSO_3_expC |
Sample type |
RNA |
|
|
Source name |
DMSO
|
Organism |
Homo sapiens |
Characteristics |
agent: DMSO gender: female developmental stage: differentiated tissue: Subcutaneous adipose tissue
|
Treatment protocol |
Compounds diluted in DMSO (0.1% final DMSO concentration), 24 hours at 37°C, 5% CO2
|
Growth protocol |
According Zen-Bio's Human Subcutaneous Adipocyte/Preadipocyte Care Manual
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the RNeasy Mini Kit (QIAGEN) according to their manual. Genomic DNA was digested on column using the DNase-Set (QIAGEN). Quality control was performed with Agilent Bioanalyser
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification Kit
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Description |
replicate 3, experiment C
|
Data processing |
The data were normalized using cubic spline normalization implemented in Illumina BeadStudio 3.3.
|
|
|
Submission date |
Apr 06, 2011 |
Last update date |
May 02, 2012 |
Contact name |
Christopher Weidner |
E-mail(s) |
weidner@molgen.mpg.de
|
Organization name |
Max Planck-Institute for Molecular Genetics
|
Department |
Otto Warburg Laboratory
|
Lab |
Sauer
|
Street address |
Ihnestraße 63
|
City |
Berlin |
ZIP/Postal code |
14195 |
Country |
Germany |
|
|
Platform ID |
GPL6947 |
Series (1) |
GSE28384 |
Amorfrutins are selective PPARγ agonists with potent antidiabetic properties |
|