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Sample GSM697328 Query DataSets for GSM697328
Status Public on Aug 01, 2011
Title Moderate 1025
Sample type RNA
 
Source name laser capture CA1 tissue gray matter from formalin fixed, paraffin embedded specimens
Organism Homo sapiens
Characteristics Sex: Male
age: 81 years old
disease status: Moderate
Extracted molecule total RNA
Extraction protocol RecoverAll Total Nucleic Acid Isolation Kit for FFPE (Ambion)
Label biotin
Label protocol 50 ng of extracted purified nucleic acid underwent RNA amplification using WT-Ovation FFPE System (NuGen) followed by FL-Ovation cDNA Biotin Module V2 (NuGen) for labeling and microarray (Affymetrix HGU133 v2) hybridization.
 
Hybridization protocol Standard Affymetrix
Scan protocol Standard Affymetrix
Description Moderate AD; correlation of the expression of each gene was tested with MiniMental Status Examination (MMSE) and neurofibrillary tangle (NFT) scores
Data processing The data were analyzed with Expression Console version 1.1 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Mar 24, 2011
Last update date Jun 26, 2019
Contact name Eric M Blalock
E-mail(s) eric.blalock@uky.edu
Phone 859-323-8033
Organization name University of Kentucky
Department Molecular and Biomedical Pharmacology
Lab Blalock
Street address 800 Rose St.
City Lexington
State/province KY
ZIP/Postal code 40475
Country USA
 
Platform ID GPL570
Series (1)
GSE28146 Microarray analyses of laser-captured hippocampus reveal distinct gray and white matter signatures associated with incipient Alzheimer’s disease
Relations
Affiliated with GSM21224
Reanalyzed by GSE133349

Data table header descriptions
ID_REF Probe Set
VALUE MAS5-calculated Signal intensity
PROBABILITY the probability that a probe set is absent, ranging from 0 to 1 (in this study, probe set with p < 0.05 were considered present)

Data table
ID_REF VALUE PROBABILITY
215306_at 129.1 0.219482
214927_at 315.3 0.046143
211606_at 9.7 0.991943
212678_at 146.4 0.27417
213929_at 59.5 0.398926
213567_at 665 0.046143
217011_at 26.3 0.981445
217483_at 312 0.111572
215769_at 143 0.466064
222271_at 69.1 0.466064
217536_x_at 1346.4 0.00293
216539_at 9.4 0.822998
216158_at 252.9 0.095215
216394_x_at 34.2 0.888428
217136_at 7.3 0.72583
214722_at 2825 0.067627
216965_x_at 16.9 0.567627
212017_at 509.5 0.219482
213392_at 4334.9 0.000244
217636_at 90 0.466064

Total number of rows: 54675

Table truncated, full table size 1343 Kbytes.




Supplementary file Size Download File type/resource
GSM697328.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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