|
| Status |
Public on May 27, 2011 |
| Title |
#757-2 [Embryonic stem cell line, ECAT11EGFP/EGFP] |
| Sample type |
RNA |
| |
|
| Source name |
#757-2 (Embryonic stem cell line, ECAT11EGFP/EGFP)
|
| Organism |
Mus musculus |
| Characteristics |
cell line: #757-2
cell type: Embryonic stem cell
strain: C57BL6/129sv origin
genotype/variation: ECAT11EGFP/EGFP Knock-in ESCs
modification: ECAT11 disrupted
gender: male
|
| Growth protocol |
Culture of ESCs in DMEM supplemented with 20% FBS, 0.1 mM non-essential amino acids, 2 mM L-glutamine, 50 U/ml penicillin-streptomycin, 0.11 mM 2-mercaptoethanol and LIF on gelatin coated dishes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Trizol
|
| Label |
Cy3
|
| Label protocol |
Agilent standard protocol
|
| |
|
| Hybridization protocol |
Agilent standard protocol
|
| Scan protocol |
Agilent standard protocol
|
| Data processing |
Data were analyzed with GeneSpring GX 11. The parameters for normalization are as follows: Data transformation: Normalize to 75th percentile
|
| |
|
| Submission date |
Mar 24, 2011 |
| Last update date |
May 27, 2011 |
| Contact name |
Shinya Yamanaka |
| E-mail(s) |
yamanaka@cira.kyoto-u.ac.jp
|
| Phone |
81-75-366-7041
|
| Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
|
| Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE28145 |
Effect of ECAT11 disruption in mouse ESCs |
|
