|
| Status |
Public on Mar 06, 2012 |
| Title |
CTR4 |
| Sample type |
RNA |
| |
|
| Source name |
retina
|
| Organism |
Homo sapiens |
| Characteristics |
retinal detachment: no
patient identifier: CTR4
total rd duration: 0 weeks
|
| Treatment protocol |
Retina samples were collected from patients with or without rhegmatogenous retinal detachment (RD)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Retinal tissue was dissected free of retinal pigment epithelium (RPE) and placed in guanidine HCL. The samples were subsequently disrupted and homogenized using a rotor–stator (Polytron PT2100). Total RNA used for microarray studies was purified from a caesium chloride gradient produced by high-speed ultra-centrifugation.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared from the total RNA according to the Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2007, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on the GeneChip Array and the GeneChips were washed and stained in the Affymetrix Fluidics Station 450 according to the standard Affymetrix protocol (Expression Analysis Technical Manual Rev. 2, 2005-2007, Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip 3000 7G scanner
|
| Description |
retina free of retinal pigment epithelium (RPE)
|
| Data processing |
GCRMA
|
| |
|
| Submission date |
Mar 23, 2011 |
| Last update date |
Mar 07, 2012 |
| Contact name |
Wolfgang Raffelsberger |
| E-mail(s) |
w.raffelsberger@unistra.fr
|
| Organization name |
IGBMC
|
| Department |
DBGS
|
| Lab |
LBGI
|
| Street address |
1 rue Laurent Fries
|
| City |
Illkirch/Strasbourg |
| ZIP/Postal code |
67404 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE28133 |
Transcriptomic analysis of human retinal detachment (RD) reveals both inflammation and photoreceptor death |
|
