 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 21, 2023 |
| Title |
pKO1_mESCs_2i_Rep2 |
| Sample type |
SRA |
| |
|
| Source name |
mESCs
|
| Organism |
Mus musculus |
| Characteristics |
cell line: R1
genotype/perturbation: Meteor promoter KO
|
| Growth protocol |
mESCs were routinely grown on gelatin-coated plates on MEFs in 2i medium: a 1:1 mixture of Neurobasal medium (Gibco) and Knockout DMEM (Gibco), supplemented with 1X B27 (Gibco), 1X N2 (Gibco), 1X Glutamax (Gibco), 1X Sodium pyruvate (Gibco), 1X Nonessential amino acids (Gibco), 0.1mM β-mercaptoethanol (Sigma), 52.5μg/ml BSA, 100U/ml Penicillin and 0.1 mg/ml Streptomycin (Biological Industries), 1μM PD 0325901 (Axon Medchem), 3μM CHIR 99021 (Axon Medchem), and 10ng/ml LIF (Weizmann Proteomics Unit). Cells were passaged with 0.25% Trypsin (Biological Industries) every 2–3 days.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted from cells using the Trizol reagent (TRI, Sigma-Aldrich) according to the manufacturer’s instructions.
Lexogen SENSE mRNA-Seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Quantification using RSEM
Assembly: mm9
Supplementary files format and content: RSEM quantification files
|
| |
|
| Submission date |
Jan 22, 2023 |
| Last update date |
Apr 21, 2023 |
| Contact name |
Igor Ulitsky |
| E-mail(s) |
igor.ulitsky@weizmann.ac.il
|
| Organization name |
Weizmann Institute of Science
|
| Street address |
Hertzl St.
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE223445 |
Complex regulation of Eomes levels mediated through distinct functional features of the Meteor long non-coding RNA locus (RNA-Seq) |
| GSE223453 |
Complex regulation of Eomes levels mediated through distinct functional features of the Meteor long non-coding RNA locus |
|
| Relations |
| BioSample |
SAMN32868338 |
| SRA |
SRX19136020 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM6956376_RSEM_pKO1_ES2i_Rep2.genes.results.gz |
449.8 Kb |
(ftp)(http) |
RESULTS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
