|
| Status |
Public on Aug 31, 2015 |
| Title |
Heart_VEHICLE_VEHICLE_Day4_Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Heart_VEHICLE_VEHICLE_Day4
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Heart
compound: Vehicle
dose: N / A
timepoint: 4 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen miRNEasy was used according to maufacturers instruction including DNAse I treatment.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Nugen protocol from 100ng total RNA using Nugen WT Ovation V2 (cat. 3100-60) and Nugen Encore Biotin (cat. 4200-60)
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.75ug of cDNA were hybridized for 18 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G.
|
| Description |
Heart from mouse treated with vehicle for 4 days (Mouse_1_Heart)
|
| Data processing |
The data were analyzed with Robust Multi-chip Average (RMA) normailzation method using Partek (Liver, heart, kidney, and skeletal muscle samples were each normalized independently)
|
| |
|
| Submission date |
Mar 15, 2011 |
| Last update date |
Aug 31, 2015 |
| Contact name |
Runxuan Zhang |
| E-mail(s) |
runxuan.zhang@hutton.ac.uk
|
| Phone |
+441382862663
|
| Organization name |
The James Hutton Institute
|
| Department |
Information and Computational Sciences
|
| Street address |
Errol Road, Invergowrie
|
| City |
Dundee |
| State/province |
Angus |
| ZIP/Postal code |
DD2 5DA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE27948 |
Pharmacology of PPAR isoform agonists on mouse |
|
