|
| Status |
Public on Jun 01, 2011 |
| Title |
I6 hESCs biological rep 1-1 [miRNA] |
| Sample type |
RNA |
| |
|
| Source name |
hESCs with SSEA4+
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
cell line: I6 hESC line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
miRNA were prepared according to the standard Agilent protocol from 100ng total RNA.
|
| |
|
| Hybridization protocol |
miRNA were hybridized for 20 hr at 55C on Agilent Human microRNA Array.Slides were washed and stained in the Agilent recommended protocol.
|
| Scan protocol |
Slides were scanned using the Agilent Scanner.
|
| Description |
HK_I6-1
miRNA expression data from SSEA4+ I6 hESC line
|
| Data processing |
The data were analyzed with Feature extraction using Agilent default analysis settings.
|
| |
|
| Submission date |
Mar 08, 2011 |
| Last update date |
Jun 01, 2011 |
| Contact name |
Jeffrey Zhao |
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Genomics Core
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6955 |
| Series (1) |
| GSE27834 |
miR-371-3 expression predicts neural differentiation potential in human pluripotent stem cells |
|
