|
| Status |
Public on Sep 15, 2011 |
| Title |
KrasTgfbr2-/- stroma specimen 113 |
| Sample type |
RNA |
| |
|
| Source name |
KrasTgfbr2-/- stroma of lung tumor , 5 weeks
|
| Organism |
Mus musculus |
| Characteristics |
strain background: LSL-KrasG12D x C57/Bl6 x Tgfbr2 flox/flox
genotype/variation: KrasTgfbr2-/-
tissue: stroma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Rneasy minikit;Qiagen,Valencia,CA
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was generated by the modified Eberwine protocol
|
| |
|
| Hybridization protocol |
cRNA was hybridized to the Affymetrix(Santa Clara,CA) 430 2.0 arrays
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from KrasTgfbr2-/- lung tumor stroma
|
| Data processing |
Array data were normalized using the RMA algorithm with R/Bioconductor as implemented in BRB-Array Tools V4.1.
|
| |
|
| Submission date |
Mar 04, 2011 |
| Last update date |
Sep 15, 2011 |
| Contact name |
Charles A Powell |
| E-mail(s) |
charles.powell@mssm.edu
|
| Fax |
212-876-5519
|
| Organization name |
Mount Sinai School of Medicine
|
| Department |
MEDICINE/PULMONARY
|
| Street address |
One Gustave Levy Place, Box 1232
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE27675 |
Expression data from lung tumor and stromal cells of KrasTgfbr2 -/- mouse model |
| GSE27719 |
Lung adenocarcinoma invasion and progression |
|
