|
| Status |
Public on Jul 07, 2006 |
| Title |
f2#202 versus pool Mouse Brain tissue |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
f2#202
|
| Organism |
Mus musculus |
| Characteristics |
Strain:C57BL/6J and C3H/HeJ on ApoE null Age:24 weeks Gender:Female
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| |
|
| Channel 2 |
| Source name |
pool
|
| Organism |
Mus musculus |
| Characteristics |
Strain:C57BL/6J and C3H/HeJ on ApoE null Age:24 weeks (150 randomly selected F2)
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| |
|
| |
| Description |
Brain tissue from 330 F2 female and male mice (animals fed a high fat "Western" diet from 8-24 weeks of age.) C57BL/J6 and C3H/HeJ on ApoE null backgrounds. All sample were compared to a common pool created from equal portions of RNA from each of the samples.
|
| Data processing |
Arrays were quantified on the basis of spot intensity relative to background (Qhyb program, Rosetta), adjusted for experimental variation between arrays using average intensity over multiple channels, and fitted to an error model to determine significance (type I error). Gene expression is reported as the ratio of the mean log10 intensity (mlratio) relative to the pool derived from 150 mice randomly selected from the F2 population. Normalized values are the reported as the ratio of the mlratio relative to the pool.
|
| |
|
| Submission date |
Aug 09, 2005 |
| Last update date |
Jul 07, 2006 |
| Contact name |
Leslie Ingram-drake |
| E-mail(s) |
lingram@mednet.ucla.edu
|
| Phone |
310-794-9036
|
| Fax |
310-794-7345
|
| URL |
http://www.mednet.ucla.edu
|
| Organization name |
UCLA
|
| Department |
Medicine/MIMG/Human Genetics
|
| Street address |
47-123 CHS
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-1359 |
| Country |
USA |
| |
|
| Platform ID |
GPL2510 |
| Series (1) |
| GSE3087 |
Expression profiling of brain tissue from (C57BL/6J X C3H/HeJ)F2 mice on ApoE null backgrounds |
|
