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Sample GSM682066 Query DataSets for GSM682066
Status Public on Mar 01, 2011
Title PBMC_malignant_validation_12
Sample type RNA
 
Source name PBMCs from patients with diagnosis of invasive breast cancer, confirmed by diagnostic biopsy
Organism Homo sapiens
Characteristics phenotype: Malignant
tissue: peripheral blood mononuclear cells
data set: validation
Extracted molecule total RNA
Extraction protocol Total RNA from peripheral blood mononuclear cells was isolated using the Ambion Ribopure™-Blood kit (Ambion, Austin, TX).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix IVT 1-cycle protocol from 2.5 ug total RNA.
 
Hybridization protocol Following fragmentation, 15ug of labeled cRNA were hybridized on HU133 Plus 2.0 GeneChips for 16 hours at 45°C. GeneChips were washed and stained using the Biomex FX liquid handler.
Scan protocol GeneChips were scanned using the Axon Genepix Scanner (Genepix 4000B).
Description Blood collection: Blood samples (8mL) collected by venipuncture into BD Vacutainer CPT Cell Preparation tubes. Samples processed within 2 hours of collection.
PBMC isolation: CPT tubes were centrifuged for 30 minutes at 2,500 rpms in a centrifuge with a swinging bucket rotor. The plasma layer was removed, and then the remaining buffy coat was poured into a 15 mL conical tube. Next, 5 mL of chilled phosphate buffered saline (PBS) with 2% fetal bovine serum (FBS) was added to the CPT tube, which was capped and inverted to mix. The contents were poured into the same 15 mL conical tube, which was then centrifuged for 10 minutes at 1200 rpms at room temperature. The supernatant was then aspirated and the pellet was resuspended in 1 mL of freezing media (RPMI with 20% DMSO and 20% FBS). The cells were then transferred to a cryovial (Nunc, Roskilde, Denmark) and placed in a freezer box at -80°C. After 24 hours samples were transferred to liquid nitrogen storage.
Data processing Data processing was performed in a pilot revision control system entitled Quality-Auditable Data Repository and Analysis (QUADRA). For fully versioned and annotated source code and data objects, please see http://quadra.genome.duke.edu/mouse-2-human-mammary-tumor-pbmc-project.
Data were RMA normalized according to the source code found at http://quadra.genome.duke.edu/mouse-2-human-mammary-tumor-pbmc-project/human-dataprep.
 
Submission date Feb 28, 2011
Last update date Nov 14, 2018
Contact name Erich Huang
E-mail(s) erich.huang@duke.edu
Phone 919-684-6849
Fax 919-681-8973
Organization name Duke University
Department Institute for Genome Sciences and Policy
Street address 101 Science Drive
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL570
Series (2)
GSE27562 Expression data from human PBMCs from breast cancer patients and controls
GSE27567 Integrating Factor Analysis and a Transgenic Mouse Model to Reveal a Peripheral Blood Predictor of Breast Tumors
Relations
Reanalyzed by GSE122511

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 8.36E+00
AFFX-BioB-M_at 9.20E+00
AFFX-BioB-3_at 8.57E+00
AFFX-BioC-5_at 9.76E+00
AFFX-BioC-3_at 1.02E+01
AFFX-BioDn-5_at 1.07E+01
AFFX-BioDn-3_at 1.24E+01
AFFX-CreX-5_at 1.31E+01
AFFX-CreX-3_at 1.34E+01
AFFX-DapX-5_at 8.87E+00
AFFX-DapX-M_at 1.03E+01
AFFX-DapX-3_at 1.09E+01
AFFX-LysX-5_at 6.09E+00
AFFX-LysX-M_at 7.32E+00
AFFX-LysX-3_at 8.30E+00
AFFX-PheX-5_at 6.95E+00
AFFX-PheX-M_at 7.28E+00
AFFX-PheX-3_at 7.67E+00
AFFX-ThrX-5_at 5.88E+00
AFFX-ThrX-M_at 6.96E+00

Total number of rows: 54675

Table truncated, full table size 1057 Kbytes.




Supplementary file Size Download File type/resource
GSM682066.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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