|
| Status |
Public on Dec 31, 2011 |
| Title |
1C5_tun |
| Sample type |
RNA |
| |
|
| Source name |
U87 glioma cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U87-MG
plasmid: pCDNA3.1_IRE1DN
treatment: upon tunicamycin treatment
|
| Treatment protocol |
cells were washed with ice cold PBS
|
| Growth protocol |
U87 control and IRE1DN cells subjected to severe hypoxia (0.1%), glucose deprivation, glutamine deprivation and tunicamycin treatment (5ug/ml) for 16h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was purified on Quiagen columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
standard Affy protocol
|
| Description |
Gene expression data from U87_IRE1DNcells upon tunicamycin treatment
|
| Data processing |
GCOS 1.2 software (Affymetrix).
|
| |
|
| Submission date |
Feb 14, 2011 |
| Last update date |
Dec 31, 2011 |
| Contact name |
eric chevet |
| E-mail(s) |
eric.chevet@u-bordeaux2.fr
|
| Organization name |
inserm
|
| Department |
U1053
|
| Street address |
146 rue Leo Saignat
|
| City |
Bordeaux |
| ZIP/Postal code |
33076 |
| Country |
France |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE27306 |
IRE1-dependent transcriptome remodelling upon ER stress in human glioma cells |
|
