|
| Status |
Public on Mar 01, 2023 |
| Title |
CUTRUN_6c_HUDEP2_H3K27me3_Cas12a CBX2-4-8 gRNA #1 transduction_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
HUDEP2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HUDEP2
cell type: Human erythroblasts
differeniation stage: undifferentiated
treatment: Cas12a CBX2-4-8 gRNA #1
chip antibody: H3K27me3(Cell signaling #9733S)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were directly used in CUT&RUN or fixed with 1% formaldehyde for 10 minutes in ChIP-seq
Sequencing libraries were generated using NEB kits (E7645 and E6440S)
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
NextSeq 2000 |
| |
|
| Description |
Rep2
|
| Data processing |
Sequencing data were analyzed by CUT&RUNTools 2.0
Peaks were called using MACS2
Assembly: hg38
Supplementary files format and content: bigWig
Library strategy: CUT&RUN
|
| |
|
| Submission date |
Nov 17, 2022 |
| Last update date |
Mar 01, 2023 |
| Contact name |
KUNHUA QIN |
| E-mail(s) |
qink@chop.edu, kunhua.qin@gmail.com
|
| Phone |
2153603146
|
| Organization name |
Children's Hospital of Philadelphia
|
| Lab |
Gerd A Blobel
|
| Street address |
3615 Civic Center Blvd, 315H
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL30173 |
| Series (2) |
| GSE218231 |
Molecular Dissection of Polycomb Group Protein-mediated Fetal Hemoglobin Repression [CUT&RUN] |
| GSE218233 |
Molecular Dissection of Polycomb Group Protein-mediated Fetal Hemoglobin Repression |
|
| Relations |
| BioSample |
SAMN31774705 |
| SRA |
SRX18297846 |
