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Sample GSM6737597 Query DataSets for GSM6737597
Status Public on Mar 01, 2023
Title CUTRUN_6c_HUDEP2_H3K27me3_Cas12a CBX2-4-8 gRNA #1 transduction_rep2
Sample type SRA
 
Source name HUDEP2
Organism Homo sapiens
Characteristics cell line: HUDEP2
cell type: Human erythroblasts
differeniation stage: undifferentiated
treatment: Cas12a CBX2-4-8 gRNA #1
chip antibody: H3K27me3(Cell signaling #9733S)
Extracted molecule genomic DNA
Extraction protocol Cells were directly used in CUT&RUN or fixed with 1% formaldehyde for 10 minutes in ChIP-seq
Sequencing libraries were generated using NEB kits (E7645 and E6440S)
 
Library strategy OTHER
Library source genomic
Library selection other
Instrument model NextSeq 2000
 
Description Rep2
Data processing Sequencing data were analyzed by CUT&RUNTools 2.0
Peaks were called using MACS2
Assembly: hg38
Supplementary files format and content: bigWig
Library strategy: CUT&RUN
 
Submission date Nov 17, 2022
Last update date Mar 01, 2023
Contact name KUNHUA QIN
E-mail(s) qink@chop.edu, kunhua.qin@gmail.com
Phone 2153603146
Organization name Children's Hospital of Philadelphia
Lab Gerd A Blobel
Street address 3615 Civic Center Blvd, 315H
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL30173
Series (2)
GSE218231 Molecular Dissection of Polycomb Group Protein-mediated Fetal Hemoglobin Repression [CUT&RUN]
GSE218233 Molecular Dissection of Polycomb Group Protein-mediated Fetal Hemoglobin Repression
Relations
BioSample SAMN31774705
SRA SRX18297846

Supplementary file Size Download File type/resource
GSM6737597_6c_S11_L001.cpm.norm.bw 132.5 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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