|
| Status |
Public on Nov 01, 2011 |
| Title |
NSVir-8d |
| Sample type |
SRA |
| |
|
| Source name |
Infected oyster hemocytes
|
| Organism |
Magallana gigas |
| Characteristics |
age: 12 months
gender: mixed
cell type: hemocytes
treatment: virulent Vibrio infection (V. splendidus LGP32 and V. aestuarianus LPi 02/41 Type I)
|
| Treatment protocol |
The DGE library NSVir-8d was constructed from Crassostrea gigas oyster hemocytes collected 8 days before an experimental infection by virulent Vibrio species (V. splendidus LGP32 and V. aestuarianus LPi 02/41 Type I). Only animals that did not survive infection were used.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
non-genomic |
| Library selection |
other |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
anchor: NlaIII
tag_length: 17
tag_count: 3866796
|
| Data processing |
The sequence files of each DGE library were analyzed with BIOTAG software (Skuldtech, Montpellier, France) (Piquemal et al. Genomics 2002) for tag detection, tag counting and for assessing DGE library quality.
|
| |
|
| Submission date |
Feb 04, 2011 |
| Last update date |
May 16, 2014 |
| Contact name |
piquemal david |
| E-mail(s) |
piquemal@skuldtech.com
|
| Organization name |
Skuld Tech
|
| Street address |
134 rue du Curat
|
| City |
Montpellier |
| ZIP/Postal code |
34090 |
| Country |
France |
| |
|
| Platform ID |
GPL9389 |
| Series (1) |
| GSE33390 |
Whole transcriptome profiling of successful immune response to Vibrio infections in the oyster Crassostrea gigas by digital gene expression analysis |
|
| Relations |
| BioSample |
SAMN02197080 |
