|
| Status |
Public on Sep 30, 2022 |
| Title |
BW25113-3-1 |
| Sample type |
SRA |
| |
|
| Source name |
Whole body
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
tissue: Whole body
treatment: fed E. coli BW25113
|
| Treatment protocol |
Caenorhabditis elegans fed by E.coli. Larva stage 4 worms were transferred to NGM containing E.coli BW25113 or purE.
|
| Growth protocol |
Worms were grown at 20℃ on nematode growth media (NGM) plates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the synchronized adult C. elegans using Trizol (Invitrogen, NO:15596026) according to the manufacturing instructions. RNA was qualified and quantified using NanoDrop bioanalyzer and Agilent 2100 (Thermo Fisher Scientific, USA).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
DNBSEQ-G400 |
| |
|
| Description |
BW25113.txt
|
| Data processing |
The quality control (QC) of the raw reads is required to determine whether the sequencing data is suitable for subsequent analysis
the filtered clean reads are aligned to the reference sequence (NCBI: GCF_000002985.6_WBcel235).
The expression levels of genes were standardized by FPKM.
Assembly: Reference Genome Version: GCF_000002985.6_WBcel235
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
Sep 27, 2022 |
| Last update date |
Sep 30, 2022 |
| Contact name |
zuobin zhu |
| E-mail(s) |
zhuzuobin@xzhmu.edu.cn
|
| Organization name |
xuzhou medical university
|
| Street address |
tongshan road 209
|
| City |
xuzhou |
| ZIP/Postal code |
221004 |
| Country |
China |
| |
|
| Platform ID |
GPL30091 |
| Series (1) |
| GSE214285 |
Insights into the genetic influences of the microbiota on the life span of a host |
|
| Relations |
| BioSample |
SAMN31037889 |
| SRA |
SRX17723588 |
