|
| Status |
Public on Jan 20, 2011 |
| Title |
Ovarian Cancer SO154 |
| Sample type |
RNA |
| |
|
| Source name |
Ovarian tumor
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Late-stage high-grade ovarian cancer
surgery outcome: Suboptimal
status: AWD (alive with disease)
survival years: 3.7
|
| Treatment protocol |
Tumor specimens were obtained from 185 previously untreated late-stage (III–IV) high-grade ovarian cancer patients. A set of 10 normal ovarian surface epithelium (OSE) cytobrushing specimens were used as control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Micro kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Labeled amplified RNA (aRNA) for microarray analysis was generated from tumor or OSE-derived total RNA using a single round of amplification
|
| |
|
| Hybridization protocol |
A 15 microgram aliquot of amplified biotinylated RNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
|
| Scan protocol |
Array images were acquired using a GeneChip Scanner 3000 (Affymetrix)
|
| Description |
Gene expression data from late-stage high-grade ovarian cancer
|
| Data processing |
Robust Multi-Array Analysis was performed
|
| |
|
| Submission date |
Jan 19, 2011 |
| Last update date |
Jan 20, 2011 |
| Contact name |
Michael Birrer |
| E-mail(s) |
mbirrer@partners.org
|
| Phone |
6177244800
|
| Organization name |
MGH
|
| Department |
Medicine
|
| Lab |
Surgical Oncology Research Labs
|
| Street address |
70 Blossom Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE26712 |
A Gene Signature Predicting for Survival in Suboptimally Debulked Patients with Ovarian Cancer |
|
