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| Status |
Public on Jun 26, 2024 |
| Title |
WT-rep-1-low_O2 |
| Sample type |
SRA |
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| Source name |
ES
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| Organism |
Homo sapiens |
| Characteristics |
tissue: ES
cell line: WA26
cell type: human ES cells
genotype: WT
treatment: 5% O2
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| Extracted molecule |
total RNA |
| Extraction protocol |
cells were flash frozen on dry ice, and RNA was harvested using RNA kit(QIAGEN,74134). Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
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| Data processing |
The quality control with the software FastQC v0.11.9
Clean reads were aligned to the human hg38 genome with the hisat2 software
The normalized counts were calculated with featurecounts
Assembly: hg38
Supplementary files format and content: counts
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| Submission date |
Sep 10, 2022 |
| Last update date |
Jun 26, 2024 |
| Contact name |
nannan zhao |
| E-mail(s) |
nnzhao@nankai.edu.cn
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| Phone |
15600911786
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| Organization name |
nankai university
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| Street address |
nankai
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| City |
tianjin |
| State/province |
--- Select a state --- |
| ZIP/Postal code |
130000 |
| Country |
China |
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| Platform ID |
GPL16791 |
| Series (1) |
| GSE213094 |
RNA-Seq of WT and UBQLN1 KO hESCs treatment with 5%O2 or NAC |
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| Relations |
| BioSample |
SAMN30790652 |
| SRA |
SRX17525438 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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