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Sample GSM650992 Query DataSets for GSM650992
Status Public on Jan 07, 2011
Title HeLa U133_Plus2 gene expression
Sample type RNA
 
Source name HeLa (ATCC CCL-2)
Organism Homo sapiens
Characteristics cell line: HeLa (ATCC CCL-2)
Treatment protocol No treatment done
Growth protocol Adherant HeLa (ATCC CCL-2) cells were maintained in DMEM/high glucose (Hyclone, Thermo Scientific) in the presence of 10% DCS and 1X penicillin/streptomycin at in 5% CO2 37 degrees.
Extracted molecule total RNA
Extraction protocol Total RNA extraction is according to the manufacturer's instructions (Invitrogen) using Trizole reagent.
Label biotin
Label protocol Samples were processed and labeled according to Affymetrix protocols.
 
Hybridization protocol Hybridizations were performed as directed by the manufacturer.
Scan protocol The microarray was scanned using a GeneChIP Scanner 3000.
Description gene expression profiling of asynchronously growing HeLa cells
Data processing The data was analyed using GeneChIP Operating Software (GCOS; Affymetrix).
Transcript signal was mapped against the chromosome coordinates (probe-by-probe basis) using the HG-U133A_2 Annotations CSV file for hg18 build of the human genome provided by Affymetrix.
 
Submission date Jan 06, 2011
Last update date Jan 07, 2011
Contact name Anindya Dutta
E-mail(s) ad8q@virginia.edu
Organization name University Of Virginia
Department Biochemistry & Molecular Genetics
Lab Dutta Lab
Street address 1300 Jefferson Park Ave
City Charlottesville
State/province VA
ZIP/Postal code 22908
Country USA
 
Platform ID GPL570
Series (1)
GSE26471 HeLa gene expression profile

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 459.064 P 0.000662269
AFFX-BioB-M_at 588.403 P 4.42873e-05
AFFX-BioB-3_at 306.044 P 8.14279e-05
AFFX-BioC-5_at 1107.15 P 4.42873e-05
AFFX-BioC-3_at 1248.25 P 4.42873e-05
AFFX-BioDn-5_at 2416.3 P 4.42873e-05
AFFX-BioDn-3_at 4807.31 P 4.42873e-05
AFFX-CreX-5_at 12070.6 P 5.16732e-05
AFFX-CreX-3_at 14871.7 P 4.42873e-05
AFFX-DapX-5_at 13.6697 A 0.455405
AFFX-DapX-M_at 4.25926 A 0.897835
AFFX-DapX-3_at 4.401 A 0.617401
AFFX-LysX-5_at 11.9678 A 0.455409
AFFX-LysX-M_at 26.065 A 0.60308
AFFX-LysX-3_at 4.46804 A 0.382599
AFFX-PheX-5_at 2.47905 A 0.804734
AFFX-PheX-M_at 2.05662 A 0.921998
AFFX-PheX-3_at 21.388 A 0.354453
AFFX-ThrX-5_at 21.9595 A 0.300591
AFFX-ThrX-M_at 2.03256 A 0.904333

Total number of rows: 54675

Table truncated, full table size 1638 Kbytes.




Supplementary file Size Download File type/resource
GSM650992.CEL.gz 4.6 Mb (ftp)(http) CEL
GSM650992.CHP.gz 6.4 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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