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Status |
Public on Jan 07, 2011 |
Title |
HeLa U133_Plus2 gene expression |
Sample type |
RNA |
|
|
Source name |
HeLa (ATCC CCL-2)
|
Organism |
Homo sapiens |
Characteristics |
cell line: HeLa (ATCC CCL-2)
|
Treatment protocol |
No treatment done
|
Growth protocol |
Adherant HeLa (ATCC CCL-2) cells were maintained in DMEM/high glucose (Hyclone, Thermo Scientific) in the presence of 10% DCS and 1X penicillin/streptomycin at in 5% CO2 37 degrees.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extraction is according to the manufacturer's instructions (Invitrogen) using Trizole reagent.
|
Label |
biotin
|
Label protocol |
Samples were processed and labeled according to Affymetrix protocols.
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|
|
Hybridization protocol |
Hybridizations were performed as directed by the manufacturer.
|
Scan protocol |
The microarray was scanned using a GeneChIP Scanner 3000.
|
Description |
gene expression profiling of asynchronously growing HeLa cells
|
Data processing |
The data was analyed using GeneChIP Operating Software (GCOS; Affymetrix). Transcript signal was mapped against the chromosome coordinates (probe-by-probe basis) using the HG-U133A_2 Annotations CSV file for hg18 build of the human genome provided by Affymetrix.
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|
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Submission date |
Jan 06, 2011 |
Last update date |
Jan 07, 2011 |
Contact name |
Anindya Dutta |
E-mail(s) |
ad8q@virginia.edu
|
Organization name |
University Of Virginia
|
Department |
Biochemistry & Molecular Genetics
|
Lab |
Dutta Lab
|
Street address |
1300 Jefferson Park Ave
|
City |
Charlottesville |
State/province |
VA |
ZIP/Postal code |
22908 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
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