|
| Status |
Public on Oct 05, 2022 |
| Title |
TFK-1, N2 |
| Sample type |
RNA |
| |
|
| Source name |
Extrahepatic cholangiocarcinoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: TFK-1
cell type: extrahepatic cholangiocarcinoma cell
genotype: Normal control
|
| Treatment protocol |
TFK-1 cells were transduced with specific lentiviruses at a multiplicity of infection (MOI) of 20 in the presence of HistransG (Genechem) for 24 h. The cells were cultured in complete medium for two days and treated with 1 μg/ml of puromycin for the establishment of stable cell lines.
|
| Growth protocol |
TFK-1 was maintained in RPMI-1640 with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from individual cell samples using RNA Extraction Reagent (Vazyme) according to the instructions. The samples were resuspended with nuclease-free water.RNA quantity and purity was assessed using NanoDrop ND-1000.
|
| Label |
Cy5
|
| Label protocol |
Target preparation was performed using an Eberwine-based amplification method with Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion, AM1753) to generate amino-allyl antisense RNA(aa-aRNA). Cy5-labeled aRNA coupled with NHS-CyDye was prepared and purified prior to hybridization. Purified coupled aRNA was quantified using NanoDrop ND-1000.
|
| |
|
| Hybridization protocol |
10 ug of Cy5-labeled aRNA was hybridized to the Phalanx Human OneArray 6.1 by Phalanx Hybridization System according to the Phalanx Hybridization Protocol.
|
| Scan protocol |
After hybridization, the arrays were washed, scanned and the gene expression results were extracted by DNA Microarray Scanner G2565B (Agilent), according to the manufacturer’s instructions.
|
| Description |
TFK-1 cells (HOXA5 overexpression VS Normal control)
|
| Data processing |
Raw fluorescence intensity values were normalized and log-transformed using GeneSpring GX 10 software (Agilent).
Expression value normalized and log-transformed by GeneSpring GX 10 software
|
| |
|
| Submission date |
Aug 23, 2022 |
| Last update date |
Oct 05, 2022 |
| Contact name |
Fei Xiong |
| E-mail(s) |
sillcon2008@hotmail.com
|
| Organization name |
Huazhong University of Science and Technology
|
| Street address |
Jiefang Road No.1095
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430074 |
| Country |
China |
| |
|
| Platform ID |
GPL16951 |
| Series (2) |
| GSE211907 |
The role HOXA5 in extrahepatic cholangiocarcinoma (Expression) |
| GSE211912 |
The role HOXA5 in extrahepatic cholangiocarcinoma |
|
