|
| Status |
Public on Feb 21, 2011 |
| Title |
Xenopus_xSGK1-morpholinos_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
xSGK1-morpholinos-injected Xenopus laevis embryos
|
| Organism |
Xenopus laevis |
| Characteristics |
tissue: whole embryo
developmental stage: stage 12
treatment: xSGK1 morpholinos
|
| Treatment protocol |
We radially injected control-morpholino (80 ng) or xSGK1-morpholinos (morpholino1 40 ng and morpholino2 40 ng) into 4-cell embryos, and cultured the embryos until stage 12.
|
| Growth protocol |
Xenopus eggs were obtained by in vitro fertilization and were dejellied in 2% cysteine, pH 7.8.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using TRIzol (Invitrogen) and purified through RNeasy columns (Qiagen), according to the manufacturers' instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix protocol (Two-Cycle Target Labeling Assays) from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees on the GeneChip Xenopus laevis Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner.
|
| Description |
xSGK1-MO2
Xenopus embryos injected with xSGK1-morpholinos.
|
| Data processing |
The data were analyzed with the MAS 5.0 algorithm using GeneChip Operating Software (GCOS) v. 1.4.
|
| |
|
| Submission date |
Dec 30, 2010 |
| Last update date |
Feb 21, 2011 |
| Contact name |
Eisuke Nishida |
| E-mail(s) |
nishida@lif.kyoto-u.ac.jp
|
| Phone |
+81-75-753-4230
|
| Organization name |
Graduate School of Biostudies, Kyoto University
|
| Department |
Department of Cell and Developmental Biology
|
| Street address |
Kitashirakawa, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1318 |
| Series (1) |
| GSE26381 |
The Kinase SGK1 in the Endoderm and Mesoderm Promotes Ectodermal Survival by Downregulating Components of the Death-Inducing Signaling Complex |
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