|
| Status |
Public on Dec 17, 2013 |
| Title |
Yap1 mutant, CHP treated, t=6 min, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Yeast, Yap1 mutant, CHP treated, t=6 min, biological replicate 1
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
agent: CHP
time point: t=6 min
strain: BY4743
genotype: yap1 mutant
growth phase: Mid-exponental phase
|
| Treatment protocol |
In mid-exponential phase (OD600 ~ 1.5), three replicate cultures were treated with 0.19 mM CHP, while three untreated cultures were used as controls.
|
| Growth protocol |
Yeast cultures were grown in controlled batch conditions, in 1 L fermentors at 30C, pH 6.0, 500 rpm, and dO2> 80%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with a procedure modified from the hot phenol protocol, as described (Martins, A. et al (2007) Yeast 24, 181-188)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast S98 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 640.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 7G Scanner.
|
| Description |
Culture treated with CHP 0.19 mM, collected at t=6 min (after adding CHP), biological replicate 1
|
| Data processing |
The data were normalized with RMA (RMAExpress version 0.5).
|
| |
|
| Submission date |
Dec 18, 2010 |
| Last update date |
Dec 17, 2013 |
| Contact name |
Pedro Mendes |
| E-mail(s) |
pedro.mendes@manchester.ac.uk
|
| Organization name |
University of Manchester
|
| Department |
Manchester Institute of Biotechnology
|
| Street address |
131 Princess Street
|
| City |
Manchester |
| ZIP/Postal code |
M1 7DN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL90 |
| Series (1) |
| GSE26169 |
Expression data for Saccharomyces cerevisiae oxidative stress response |
|
