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Status |
Public on Dec 17, 2013 |
Title |
Gpx2 mutant, Control, t=20 min, biological replicate 2 |
Sample type |
RNA |
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Source name |
Yeast, Gpx2 mutant, Control, t=20 min, biological replicate 2
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Organism |
Saccharomyces cerevisiae |
Characteristics |
agent: Control time point: t=20 min strain: BY4743 genotype: gpx2 mutant growth phase: Mid-exponental phase
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Treatment protocol |
In mid-exponential phase (OD600 ~ 1.5), three replicate cultures were treated with 0.19 mM CHP, while three untreated cultures were used as controls.
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Growth protocol |
Yeast cultures were grown in controlled batch conditions, in 1 L fermentors at 30C, pH 6.0, 500 rpm, and dO2> 80%.
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with a procedure modified from the hot phenol protocol, as described (Martins, A. et al (2007) Yeast 24, 181-188)
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast S98 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 640.
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Scan protocol |
GeneChips were scanned using the Affymetrix 7G Scanner.
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Description |
Control (untreated culture), collected at t=20 min (after adding CHP to treated cultures), biological replicate 2
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Data processing |
The data were normalized with RMA (RMAExpress version 0.5).
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Submission date |
Dec 18, 2010 |
Last update date |
Dec 17, 2013 |
Contact name |
Pedro Mendes |
E-mail(s) |
pedro.mendes@manchester.ac.uk
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Organization name |
University of Manchester
|
Department |
Manchester Institute of Biotechnology
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Street address |
131 Princess Street
|
City |
Manchester |
ZIP/Postal code |
M1 7DN |
Country |
United Kingdom |
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Platform ID |
GPL90 |
Series (1) |
GSE26169 |
Expression data for Saccharomyces cerevisiae oxidative stress response |
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