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Sample GSM642513 Query DataSets for GSM642513
Status Public on Dec 17, 2013
Title Gpx2 mutant, Control, t=20 min, biological replicate 2
Sample type RNA
 
Source name Yeast, Gpx2 mutant, Control, t=20 min, biological replicate 2
Organism Saccharomyces cerevisiae
Characteristics agent: Control
time point: t=20 min
strain: BY4743
genotype: gpx2 mutant
growth phase: Mid-exponental phase
Treatment protocol In mid-exponential phase (OD600 ~ 1.5), three replicate cultures were treated with 0.19 mM CHP, while three untreated cultures were used as controls.
Growth protocol Yeast cultures were grown in controlled batch conditions, in 1 L fermentors at 30C, pH 6.0, 500 rpm, and dO2> 80%.
Extracted molecule total RNA
Extraction protocol RNA was extracted with a procedure modified from the hot phenol protocol, as described (Martins, A. et al (2007) Yeast 24, 181-188)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Yeast S98 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 640.
Scan protocol GeneChips were scanned using the Affymetrix 7G Scanner.
Description Control (untreated culture), collected at t=20 min (after adding CHP to treated cultures), biological replicate 2
Data processing The data were normalized with RMA (RMAExpress version 0.5).
 
Submission date Dec 18, 2010
Last update date Dec 17, 2013
Contact name Pedro Mendes
E-mail(s) pedro.mendes@manchester.ac.uk
Organization name University of Manchester
Department Manchester Institute of Biotechnology
Street address 131 Princess Street
City Manchester
ZIP/Postal code M1 7DN
Country United Kingdom
 
Platform ID GPL90
Series (1)
GSE26169 Expression data for Saccharomyces cerevisiae oxidative stress response

Data table header descriptions
ID_REF
VALUE Log2 RMA signal

Data table
ID_REF VALUE
10000_at 2.896478
10001_at 7.586071
10002_i_at 9.511968
10003_f_at 11.952547
10004_at 3.089835
10005_at 7.330732
10006_at 6.145103
10007_at 8.43354
10008_at 4.878713
10009_at 5.193706
10010_at 10.23177
10011_at 3.603952
10012_at 4.10438
10013_at 4.958275
10014_at 7.885593
10015_at 11.090821
10016_at 9.709644
10017_at 4.152065
10018_at 12.21201
10019_at 11.663032

Total number of rows: 9335

Table truncated, full table size 159 Kbytes.




Supplementary file Size Download File type/resource
GSM642513.CEL.gz 1.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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