|
| Status |
Public on Dec 04, 2016 |
| Title |
ChIP_SRC1_Veh_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Cell line
|
| Organism |
Homo sapiens |
| Characteristics |
strain: U2OS-ERalpha (stable)
antibody: anti-SRC1 M341
antibody vendor, catalog#: Santa Cruz, sc-8995
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Purified DNA given A overhang by Klenow exo, adapters ligated, gel size selected (300±25bp), PCR amplified and sequenced
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
Reads are mapped to genome using ELAND. Reads that are uniquely mapped and have no more than 2 mismatches are selected for future analysis. MACS is used to call peaks with p value 1e-10.
|
| |
|
| Submission date |
Dec 16, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
kaifu chen |
| E-mail(s) |
kaifuc@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Street address |
1 Baylor plaza, suite 450A
|
| City |
Houston |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL9115 |
| Series (1) |
| GSE26110 |
Genome-wide analysis of SRC-1/NCOA1 and Erα/ESR1 binding in chromatin obtained from the ERα stably transfected U2OS osteosarcoma cell line (U2OS-ERα) with ChIP-SEQ. |
|
| Relations |
| SRA |
SRX036640 |
| BioSample |
SAMN00189072 |
