|
| Status |
Public on Oct 25, 2011 |
| Title |
Casting-Treatment-Day2-Rep7 |
| Sample type |
RNA |
| |
|
| Source name |
Gastroc Muscle, Casting, Day 2
|
| Organism |
Mus musculus |
| Characteristics |
gender: Male
animal number: P-09
tissue: Muscle;Gastrocnemius
tissue location: Right
strain: C57BL/6
treatment: Casting
timepoint: Day 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA followed by RNEasy purification was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip MOE430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were analyzed with Microarray Suite GCOS using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Dec 07, 2010 |
| Last update date |
Oct 25, 2011 |
| Contact name |
Scott Jelinsky |
| E-mail(s) |
Scott.Jelinsky@pfizer.com
|
| Phone |
617-674-7272
|
| Organization name |
Pfizer
|
| Department |
Inflammation and Immunology
|
| Lab |
Computational Precision Medicine
|
| Street address |
610 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE25908 |
Distinct Protein Degradation Induced by Different Disuse Models of Skeletal Muscle Atrophy |
|
