|
| Status |
Public on Dec 03, 2010 |
| Title |
4.2_B_2_2 |
| Sample type |
RNA |
| |
|
| Source name |
bystander fibroblast 4 h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: immortalized hTERT
cell type: bystander fibroblast
time point: 4 h
|
| Treatment protocol |
Irradiated cells were irradiated with 2.0 Gy of gamma radiation. Control cells were incubated in medium from non-irraidated cells 2 h after irradiation. Bystander cells were incubated with medium from cells irradiated with 2.0 Gy, 2 h after irradiation. Extraction of RNA was done 4, 6 and 8 h after irradiation.
|
| Growth protocol |
The cells were grown in DMEM supplied with 1% L-Glutamine and Penicillin/ Streptomycin and 20% FBS and kept at 37°C and 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from 4 independent experiments was isolated from cells irradiated with 2.0 Gy and bystander fibroblasts 4, 8 and 26 h after irradiation using Pure Link RNA mini kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.
|
| Label |
biotin, Cy3
|
| Label protocol |
Performed with Illumina® TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
performed with Illumina BeadStudio 3.1.3.0 Gene Expression Module 3.4.0
|
| |
|
| Submission date |
Dec 02, 2010 |
| Last update date |
Dec 02, 2010 |
| Contact name |
Erta kalanxhi |
| E-mail(s) |
erta.kalanxhi@rr-research.no
|
| Organization name |
Oslo University Hospital
|
| Street address |
Montebello 0310
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE25772 |
Genome-wide microarray analysis of normal human fibroblasts in response to γ-radiation and the radiation induced bystander effect |
|
