|
Status |
Public on Jul 10, 2012 |
Title |
T_cell |
Sample type |
RNA |
|
|
Source name |
Purified cells, tonsil, T cells
|
Organism |
Homo sapiens |
Characteristics |
tissue: tonsil cell type: T cells disease status: healthy control
|
Growth protocol |
Frozen biopsies from previously untreated patients for lymphoma samples and purified populations from tonsil or peripheral blood for normal controls.
|
Extracted molecule |
total RNA |
Extraction protocol |
The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
|
Label |
biotin
|
Label protocol |
The cDNA was prepared from 5 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affimetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affimetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133_Plus2 chip according to the protocols provided by the manufacturer.
|
Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
|
Data processing |
RMA (R/Bioconductor processing)
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|
|
Submission date |
Nov 28, 2010 |
Last update date |
Jul 10, 2012 |
Contact name |
Victor Segura |
E-mail(s) |
vsegura@unav.es
|
Organization name |
FIMA
|
Department |
Unit of Proteomics, Genomics and Bioinformatics
|
Street address |
Avda. Pío XII, 55
|
City |
Pamplona |
State/province |
Navarra |
ZIP/Postal code |
31008 |
Country |
Spain |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE25638 |
A mouse model of deregulation of the malt1 oncogene recapitulates the pathogenesis of human malt lymphoma [MALT dataset] |
GSE25639 |
A mouse model of deregulation of the malt1 oncogene recapitulates the pathogenesis of human malt lymphoma |
|