|
| Status |
Public on Nov 23, 2010 |
| Title |
CD16 Control |
| Sample type |
RNA |
| |
|
| Source name |
unstimulated CD14loCD16+ monocytes from peripheral human blood
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD14loCD16+ monocytes isolated from peripheral human blood by CD16 microbeads
|
| Biomaterial provider |
Lab D3, University of Texas Health Scienece Center at Tyler
|
| Treatment protocol |
untreated
|
| Growth protocol |
CD14loCD16+ monocytes were isolated from human peripheral blood using magnetic beads for CD16. 1.5 million cells were cultured in 1 ml of complete RPMI medium with 10% human serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 48 hours stimulated or control CD14loCD16+ monocytes were harvested and RNA was isolated using Trizol (Invitrogen) acc. to manufacturer's protocol.
|
| Label |
Cy5
|
| Label protocol |
See http://www.phalanxbiotech.com/ for updated protocols
|
| |
|
| Hybridization protocol |
See http://www.phalanxbiotech.com/ for updated protocols
|
| Scan protocol |
See http://www.phalanxbiotech.com/ for updated protocols
|
| Data processing |
See http://www.phalanxbiotech.com/ for updated protocols and additional information
|
| |
|
| Submission date |
Nov 22, 2010 |
| Last update date |
Nov 22, 2010 |
| Contact name |
Rohan Dhiman |
| E-mail(s) |
rohandhiman79@yahoo.com
|
| Phone |
903-8775385
|
| Fax |
903-8777989
|
| Organization name |
University of Texas Health Science Center at Tyler
|
| Department |
Centre for Pulmonary and Infectious Disease Control
|
| Lab |
D3
|
| Street address |
11937 US Hwy 271
|
| City |
Tyler |
| State/province |
Texas |
| ZIP/Postal code |
75708 |
| Country |
USA |
| |
|
| Platform ID |
GPL6254 |
| Series (1) |
| GSE25505 |
Gene expression analysis of gamma-M.tuberculosis-stimulated CD14hiCD16- and CD14loCD16+ monocytes |
|
