|
| Status |
Public on Nov 30, 2011 |
| Title |
Feproexpress AP 12 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Sus scrofa |
| Characteristics |
breed: German Landrace
tissue: liver
ontogenetic stage: 94 dpc
gestation diet: offspring of German Landrace gilts fed a gestational adequate protein diet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRI Reagent (Sigma, Taufkirchen, Germany) according to the manufacturer’s protocol. After DNaseI treatment the RNA was cleaned up using the RNeasy Kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.5 ug total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42C on Affymetrix GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
|
| Description |
Gene expression data from liver sample of offspring
|
| Data processing |
Data were analysed with Affymetrix GCOS 1.1.1 software using global scaling to a target signal of 500 and BioConductor.
|
| |
|
| Submission date |
Nov 18, 2010 |
| Last update date |
Nov 30, 2011 |
| Contact name |
Klaus Wimmers |
| E-mail(s) |
s.wimmers@fbn-dummerstorf.de
|
| Phone |
0049 3820868700
|
| Organization name |
Leibniz Institute for Farm Animal Biology (FBN)
|
| Street address |
Wilhelm-Stahl-Allee 2
|
| City |
Dummerstorf |
| ZIP/Postal code |
18196 |
| Country |
Germany |
| |
|
| Platform ID |
GPL3533 |
| Series (2) |
| GSE25482 |
Fetal programming of hepatic transcriptome in response to gestational dietary protein levels in the pig (AP data set) |
| GSE25484 |
Fetal programming of hepatic transcriptome in response to gestational dietary protein levels in the pig |
|
