|
| Status |
Public on Nov 10, 2023 |
| Title |
LCZ9 Ctrl ImCD8 thymocyte |
| Sample type |
SRA |
| |
|
| Source name |
Thymus
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Thymus
cell type: ImCD8
genotype: Zfp148 fl/fl; Zfp281 fl/fl; Cd4-Cre+
flow markers: CD44low, CD8+ CD4-, TCRb+, CD69+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeasy Plus Micro kit (Qiagen)
Clontech SMARTer Low Input mRNA following the manufacturer's protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Filtering with Cutadapt 1.18
Alignment ot mm10 with STAR 2.6.1
Read counting with HTseq
Assembly: mm10
Supplementary files format and content: Htseq count table
|
| |
|
| Submission date |
Jun 21, 2022 |
| Last update date |
Nov 10, 2023 |
| Contact name |
Remy Bosselut |
| E-mail(s) |
bosselur@helix.nih.gov
|
| Phone |
240-760-6866
|
| Organization name |
National Cancer Institute, National Institute of Health
|
| Department |
Laboratory of Immune Cell Biology
|
| Lab |
Bosselut
|
| Street address |
9000 Rockville Pike, Building 37
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL21103 |
| Series (2) |
| GSE206542 |
The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Population RNA-seq] |
| GSE207572 |
Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions |
|
| Relations |
| BioSample |
SAMN29535819 |
| SRA |
SRX16041383 |
