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| Status |
Public on Jan 01, 2024 |
| Title |
KO, Spleen, 1- 1 |
| Sample type |
SRA |
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| Source name |
Spleen
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| Organism |
Mus musculus |
| Characteristics |
cell type: T cell
tissue: Spleen
strain: C57BL/6
age: 9w
treatment: o-transfer cKO CD8+ T cells
infection: Lymphocytic choriomeningitis virus Clone 13 strain
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| Extracted molecule |
total RNA |
| Extraction protocol |
Lung and spleen of co-transfer WT and cKO CD8+ T cells were purified by FACS sorting.
Single-cell cDNA libraries were prepared using the MARS-Seq method, as described (Jaitin et al., 2014; Keren-Shaul et al., 2019)
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
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| Description |
Mars-seq
cell_metadata.csv
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| Data processing |
ScRNA-seq libraries (pooled at equimolar concentrations) were sequenced on an Illumina NextSeq 500.MARS-seq reads were processed using the MARS-seq2.0 pipeline (Keren-Shaul et al., 2019).
Assembly: mm10
Supplementary files format and content: Tab-separated values files and matrix files
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| Submission date |
Jun 14, 2022 |
| Last update date |
Jan 01, 2024 |
| Contact name |
Guo Fu |
| E-mail(s) |
guofu@xmu.edu.cn
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| Organization name |
Xiamen University
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| Street address |
No. 422, Siming South Road, Xiamen, Fujian, China.
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| City |
Xiamen |
| ZIP/Postal code |
361005 |
| Country |
China |
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| Platform ID |
GPL19057 |
| Series (1) |
| GSE206067 |
Themis directs T cell exhaustion by promoting PD-1 signaling and key transcription factors TCF-1 and TOX expression |
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| Relations |
| BioSample |
SAMN29038952 |
| SRA |
SRX15697612 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM6241252_ABBB31.txt.gz |
771.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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