|
| Status |
Public on Mar 25, 2024 |
| Title |
CTRL-1_S21 |
| Sample type |
SRA |
| |
|
| Source name |
oligodendrocyte
|
| Organism |
Mus musculus |
| Characteristics |
sample group: Control
age: p5 pups
genotype: Brg1 fl/+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from control and Brg1 mutant oligodendrocytes were extracted using TRIZOL (Life Technologies) followed by purification using an RNeasy Mini Kit (Qiagen).
RNA-seq libraries were prepared using Illumina RNA-Seq Preparation Kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Illumina Casava software used for basecalling.
Sequenced reads were trimmed for adaptor sequence.
Reads mapped to mm10 whole genome by Tophat with default options
Fragment Per Kilobase of exon per Megabase of library size (FPKM) were calculated using Cufflinks with default options. Outputs from Cufflinks are provided as supplementary files as a tab-delimited text file.
Assembly: mm10
Supplementary files format and content: format:xls ; Contain the estimated expression values produced by Cufflinks.
|
| |
|
| Submission date |
Jun 01, 2022 |
| Last update date |
Mar 25, 2024 |
| Contact name |
Richard Lu |
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
CBDI
|
| Lab |
Lu Lab,T6.525
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE205236 |
BRG1 programs PRC2-complex repression and controls oligodendrocyte differentiation and remyelination |
|
| Relations |
| BioSample |
SAMN28797799 |
| SRA |
SRX15553877 |
