|
Status |
Public on Nov 04, 2010 |
Title |
iodine-deficient diet and normal water B9 |
Sample type |
RNA |
|
|
Source name |
thyroid after 2 months 1 day iodine deficient diet
|
Organism |
Rattus norvegicus |
Characteristics |
gender: male strain: Sprague-Dawley tissue: thyroid protocol: iodine-deficient diet and normal water time: 1 day
|
Treatment protocol |
Adult rats were either fed a normal diet and normal water (controls), normal diet and perchlorate treated water, iodine-deficient diet and normal water, or iodine-deficient diet and perchlorate treated water
|
Growth protocol |
Adult rats were housed in standard rat cages in the Uiversity of Georgia's animal care facility for 2 months and one day, or 2 months and 14 days, respectively.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted from thyroids using a Trizol procedure, and subsecuently cleaned using a Qiagen Rneasy column
|
Label |
biotin
|
Label protocol |
Labeling, hybing, and scanning of the chips followed standard Affymetrix protocols for gene expression assay
|
|
|
Hybridization protocol |
standard Affymetrix protocols
|
Scan protocol |
standard Affymetrix protocols
|
Description |
S805368.J05.CEL iodine deficiency
|
Data processing |
Data from the CHP file (from Affymetrix GCOS software) was statistically analyzed in GeneSpring
|
|
|
Submission date |
Nov 04, 2010 |
Last update date |
Nov 04, 2010 |
Contact name |
Ken Jones |
E-mail(s) |
kenjones@uga.edu
|
Organization name |
University of Georgia
|
Department |
Environmental Health Science
|
Street address |
150 E. Green St.
|
City |
Athens |
State/province |
GA |
ZIP/Postal code |
30602 |
Country |
USA |
|
|
Platform ID |
GPL1355 |
Series (1) |
GSE25144 |
Comparison of effects of Iodine-deficiency and perchlorate on thyroid |
|