|
| Status |
Public on Nov 04, 2010 |
| Title |
iodine-deficient diet and normal water B9 |
| Sample type |
RNA |
| |
|
| Source name |
thyroid after 2 months 1 day iodine deficient diet
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: male
strain: Sprague-Dawley
tissue: thyroid
protocol: iodine-deficient diet and normal water
time: 1 day
|
| Treatment protocol |
Adult rats were either fed a normal diet and normal water (controls), normal diet and perchlorate treated water, iodine-deficient diet and normal water, or iodine-deficient diet and perchlorate treated water
|
| Growth protocol |
Adult rats were housed in standard rat cages in the Uiversity of Georgia's animal care facility for 2 months and one day, or 2 months and 14 days, respectively.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from thyroids using a Trizol procedure, and subsecuently cleaned using a Qiagen Rneasy column
|
| Label |
biotin
|
| Label protocol |
Labeling, hybing, and scanning of the chips followed standard Affymetrix protocols for gene expression assay
|
| |
|
| Hybridization protocol |
standard Affymetrix protocols
|
| Scan protocol |
standard Affymetrix protocols
|
| Description |
S805368.J05.CEL
iodine deficiency
|
| Data processing |
Data from the CHP file (from Affymetrix GCOS software) was statistically analyzed in GeneSpring
|
| |
|
| Submission date |
Nov 04, 2010 |
| Last update date |
Nov 04, 2010 |
| Contact name |
Ken Jones |
| E-mail(s) |
kenjones@uga.edu
|
| Organization name |
University of Georgia
|
| Department |
Environmental Health Science
|
| Street address |
150 E. Green St.
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE25144 |
Comparison of effects of Iodine-deficiency and perchlorate on thyroid |
|
