|
| Status |
Public on Oct 31, 2023 |
| Title |
Brd9_KO_03 |
| Sample type |
SRA |
| |
|
| Source name |
Lineage- bone marrow cells in femora and tibiae
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: CD45.2(+) Mx1-Cre Brd9fl/fl
timing of preparation: Eight weeks after pIpC injection
|
| Treatment protocol |
Four weeks after BMT, the recipient mice received 20 mg/kg pIpC injection every other day for a total of 3 doses.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy columns (Qiagen) per manufacturer’s instructions.
RNA was poly(A)-selected, and unstranded Illumina libraries were prepared with the standard TruSeq protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Raw FASTQs were processed using nf-core/rnaseq (3.0).
Assembly: mm10
Supplementary files format and content: txt
|
| |
|
| Submission date |
May 18, 2022 |
| Last update date |
Oct 31, 2023 |
| Contact name |
Masaki Nomura |
| E-mail(s) |
nomura.masaki.7rk@osaka-u.ac.jp
|
| Organization name |
Graduate School of Medicine, The University of Osaka
|
| Department |
Department of Cancer Pathology
|
| Street address |
2-2, Yamadaoka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE203316 |
BRD9 determines the cell fate of hematopoietic stem cells by regulating chromatin state [bulk RNA-Seq I] |
| GSE203322 |
BRD9 determines the cell fate of hematopoietic stem cells by regulating chromatin state |
|
| Relations |
| BioSample |
SAMN28534445 |
| SRA |
SRX15336991 |
