|
| Status |
Public on Nov 18, 2010 |
| Title |
2 Wild-type control macrophage+RSG |
| Sample type |
RNA |
| |
|
| Source name |
Wild-type bone marrow-derived macrophage treated with RSG
|
| Organism |
Mus musculus |
| Characteristics |
mouse: 2
strain: C57Bl/6
genotype/variation: Wild-type
cell type: bone marrow-derived macrophage
activation: Control
treatment: Rosiglitazone
time: 10 days
|
| Treatment protocol |
Cell were treated after plating into 6-well cell culture plates with the indicated cytokines (20 ng/ml M-CSF. 20 ng/ml IL-4 and vehicle (DMSO:ethanol) or 1 uM Rosiglitazone.
|
| Growth protocol |
Bone marrow cells were isolated from the femur, washed twice with PBS and cultured in RPMI+10% FCS supplemented with glutamine, penicillin and streptomycin and 20 ng/ml M-CSF. Fresh media and cytokines were added every other day for 10 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on Mouse Genome 430 2.0 Affymetrix microarrays according to Affymetric standardized protocol.
|
| Scan protocol |
Microarrays were scanned canned according to Affymetric standardized protocol.
|
| Description |
Gene expression data of RSG-treated bone marrow-derived macrophages derived from C57Bl/6 wild-type mouse
6_(Mouse430_2).CEL
|
| Data processing |
The data were generated with GCOS, and cel files were analyzed in GeneSpring 7.3 using GC-RMA.
|
| |
|
| Submission date |
Nov 02, 2010 |
| Last update date |
Nov 18, 2010 |
| Contact name |
Attila Szanto |
| E-mail(s) |
attila.szanto@gmail.com
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Molecular Biology
|
| Lab |
Lee Lab
|
| Street address |
185 Cambridge St.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02446 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE16387 |
Licensing of PPARg-regulated gene expression by IL-4-induced alternative macrophage activation |
| GSE25088 |
PPARg and IL-4-induced gene expression data from wild-type and STAT6 knockout mouse bone marrow-derived macrophages |
|
