|
| Status |
Public on Oct 30, 2010 |
| Title |
Jurkat |
| Sample type |
SRA |
| |
|
| Source name |
Leukemic T-cell line originally derived from a T-ALL patient
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Leukemic T-cell
chip antibody: anti-TAL1 Ab (Santa Cruz sc-12984)
|
| Treatment protocol |
Jurkat cells were cultured in RPMI 1640 supplemented with 10% (v/v) Tet-free FBS, 100 U/ml penicillin, 100mg/ml streptomycin and 20µg/ml blasticidin.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIPed DNA was prepared according to the Illumina protocol with two modifications: 1) DNA fragments ranging from 150bp to 300bp were selected at the gel selection step; 2) 21 instead of 18 cycles of PCR were done at the amplification step.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Jurkat cells were cultured in RPMI 1640 supplemented with 10% (v/v) Tet-free FBS, 100 U/ml penicillin, 100mg/ml streptomycin and 20µg/ml blasticidin.
|
| Data processing |
Sequence reads were obtained using the Illumina Genome Analyzer II. Reads were aligned to human genome (hg18) using MAQ (version 0.6.6) .
|
| |
|
| Submission date |
Oct 28, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Stephen Tapscott |
| E-mail(s) |
stapscot@fredhutch.org
|
| Organization name |
Fred Hutch Cancer Research Center
|
| Department |
Human Biology
|
| Lab |
Tapscott
|
| Street address |
1100 Fairview N. Ave
|
| City |
Seattle |
| State/province |
WASHINGTON |
| ZIP/Postal code |
98103 |
| Country |
USA |
| |
|
| Platform ID |
GPL9052 |
| Series (1) |
| GSE25000 |
Differential genomic targeting of the transcription factor TAL1 in alternate hematopoietic lineages |
|
| Relations |
| Reanalyzed by |
GSE64832 |
| SRA |
SRX029597 |
| BioSample |
SAMN00116838 |
