NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM6071099 Query DataSets for GSM6071099
Status Public on Sep 14, 2023
Title EHMT1-K450RK451R, Veh-rep 2
Sample type SRA
 
Source name LNCaP cell
Organism Homo sapiens
Characteristics cell type: prostate cancer cell
cell line: LNCaP-tet-EHMT1
treatment: untreated
Growth protocol In RPMI 1640 medium with 10% FBS
Extracted molecule total RNA
Extraction protocol RNA was extracted with Rneasy Mini Kit (QIAGEN) following manufacturer’s protocol.
RNA-seq libraries of both LNCaP-tet-EHMT1 and LNCaP cells were performed by using TruSeq® Strnd Total RNA LT (Illumina).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description LNCaP_EHMT1_count_table.txt.gz
Data processing The sequence quality of raw reads was checked by FastQC (version 0.11.9).
Raw reads were aligned to the human reference genome (hg19) using STAR (version 2.7.2) followed by counting with featureCounts (version 1.6.4) from GRCh37 Ensembl reference.
Assembly: hg19
Supplementary files format and content: txt file for raw counts of sequence reads
 
Submission date Apr 28, 2022
Last update date Sep 14, 2023
Contact name Changmeng Cai
E-mail(s) changmeng.cai@umb.edu
Organization name University of Massachusetts Boston
Street address 100 William T Morrissey Blvd
City Boston
State/province MA
ZIP/Postal code 02125
Country USA
 
Platform ID GPL16791
Series (2)
GSE201770 Lysine methylation of EHMT1/GLP as a molecular switch to reprogram transcription networks in prostate cancer [RNA-seq]
GSE201771 Lysine methylation of EHMT1/GLP as a molecular switch to reprogram transcription networks in prostate cancer
Relations
BioSample SAMN27926425
SRA SRX15038603

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap