|
| Status |
Public on Sep 14, 2023 |
| Title |
EHMT1-K450RK451R, Veh-rep 2 |
| Sample type |
SRA |
| |
|
| Source name |
LNCaP cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: prostate cancer cell
cell line: LNCaP-tet-EHMT1
treatment: untreated
|
| Growth protocol |
In RPMI 1640 medium with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Rneasy Mini Kit (QIAGEN) following manufacturer’s protocol.
RNA-seq libraries of both LNCaP-tet-EHMT1 and LNCaP cells were performed by using TruSeq® Strnd Total RNA LT (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
LNCaP_EHMT1_count_table.txt.gz
|
| Data processing |
The sequence quality of raw reads was checked by FastQC (version 0.11.9).
Raw reads were aligned to the human reference genome (hg19) using STAR (version 2.7.2) followed by counting with featureCounts (version 1.6.4) from GRCh37 Ensembl reference.
Assembly: hg19
Supplementary files format and content: txt file for raw counts of sequence reads
|
| |
|
| Submission date |
Apr 28, 2022 |
| Last update date |
Sep 14, 2023 |
| Contact name |
Changmeng Cai |
| E-mail(s) |
changmeng.cai@umb.edu
|
| Organization name |
University of Massachusetts Boston
|
| Street address |
100 William T Morrissey Blvd
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02125 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (2) |
| GSE201770 |
Lysine methylation of EHMT1/GLP as a molecular switch to reprogram transcription networks in prostate cancer [RNA-seq] |
| GSE201771 |
Lysine methylation of EHMT1/GLP as a molecular switch to reprogram transcription networks in prostate cancer |
|
| Relations |
| BioSample |
SAMN27926425 |
| SRA |
SRX15038603 |
