|
| Status |
Public on Jun 15, 2011 |
| Title |
4C WT mice Rad23a fetal liver replicate 1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
WT genomic DNA fetal liver, digested with HindIII and DpnII
|
| Organism |
Mus musculus |
| Characteristics |
sample type: WT genomic DNA fetal liver, digested with HindIII and DpnII
genetic background: FVB
tissue: fetal liver
genotype: wild type
age: E14.5
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
4C was performed as described before in Simonis et al. 2006 Nuclear organization of active and inactive chromatin domains uncovered by chromosome conformation capture-on-chip (4C). Nat Genet. 38(11):1348-54
|
| Label |
Cy3
|
| Label protocol |
Details on the protocol can be found on http://www.nimblegen.com/technology/index.html
|
| |
|
| Channel 2 |
| Source name |
Rad23a 4C fetal liver, digested with HindIII and DpnII
|
| Organism |
Mus musculus |
| Characteristics |
sample type: Rad23a 4C fetal liver, digested with HindIII and DpnII
genetic background: FVB
tissue: fetal liver
genotype: wild type
age: E14.5
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
4C was performed as described before in Simonis et al. 2006 Nuclear organization of active and inactive chromatin domains uncovered by chromosome conformation capture-on-chip (4C). Nat Genet. 38(11):1348-54
|
| Label |
Cy5
|
| Label protocol |
Details on the protocol can be found on http://www.nimblegen.com/technology/index.html
|
| |
|
| |
| Hybridization protocol |
Details on the protocol can be found on http://www.nimblegen.com/technology/index.html
|
| Scan protocol |
Details on the protocol can be found on http://www.nimblegen.com/technology/index.html
|
| Data processing |
Data were normalized using the 'lowess' method using standard settings (see Smyth & Speed, 2003, Methods 31, 265-273 (2003)
|
| |
|
| Submission date |
Oct 11, 2010 |
| Last update date |
Jun 15, 2011 |
| Contact name |
Elzo de Wit |
| Phone |
+31 30 2121 800
|
| Organization name |
Hubrecht Institute
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| ZIP/Postal code |
3584 CT |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL4344 |
| Series (1) |
| GSE24614 |
Variegated gene expression caused by cell-specific long-range DNA interactions |
|
