|
| Status |
Public on May 23, 2011 |
| Title |
Overexpression empty vector control for FT2 leaves, Rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
leaves, pBI101 control
|
| Organism |
Populus tremula x Populus alba |
| Characteristics |
strain: 717
tissue: leaf
age: 1-2 years
treatment: control
|
| Treatment protocol |
Treatments represent the FT1 and FT2 under both constitutive overexpression and heat shock promotion, as well as the respective controls.
|
| Growth protocol |
For 35S samples, leaf tissues were collected at approximately 9 AM. For HSP promotion treatments, leaf samples were harvested at day 21 before the end of heat shock treament. All plants were of the approximate same developmental stage and were about 1 to 2 years old.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with a hot-borate method (Wan and Wilkins; 1994), followed by a Dnase I digestion and clean-up using the Rneasy Mini Kit (Quigen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on a GeneChip Poplar Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using a GeneChip Scanner 3000 (Affymetrix).
|
| Description |
35S-WT2-3
Gene expression of leaf tissue.
|
| Data processing |
Pre-processing was done using the GCRMA algorithm in the R and Bioconductor packages.
|
| |
|
| Submission date |
Oct 08, 2010 |
| Last update date |
May 26, 2011 |
| Contact name |
Joshua Adams |
| E-mail(s) |
AdamsJ@uamont.edu
|
| Phone |
8704601348
|
| Organization name |
University of Arkansas-Monticello
|
| Department |
Forest Resources
|
| Street address |
PO Box 3468
|
| City |
Monticello |
| State/province |
AR |
| ZIP/Postal code |
71656 |
| Country |
USA |
| |
|
| Platform ID |
GPL4359 |
| Series (1) |
| GSE24609 |
FT1 and FT2 network analysis in poplar |
|
