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Status
Public on Oct 08, 2010
Title
DC.8-4-11b-.MLN#3
Sample type
RNA
Source name
mesenteric lymph node
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Blendzyme liberase III + DNase digestion, CD3, CD19, NK1.1, GR1, Ter119 MACS depletion
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
DC.8-4-11b-.MLN#3, CD11c+, CD8a-, CD4-, CD11b-
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Oct 07, 2010
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
15.7063
10344622
201.8242
10344624
347.5046
10344633
997.7087
10344637
255.8605
10344653
16.2091
10344658
189.5389
10344674
11.2057
10344679
47.1212
10344707
231.4153
10344713
184.6188
10344723
75.6565
10344725
38.7186
10344741
2259.1769
10344743
28.8065
10344750
642.6029
10344772
33.9607
10344789
150.0105
10344797
360.0594
10344799
168.9191
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM605835_EA07068_91090_MoGene_DC4.MLN#3.CEL.gz
4.5 Mb
(ftp) (http) CEL
Processed data included within Sample table
