|
| Status |
Public on Jan 20, 2012 |
| Title |
Tumor from ApcD14/+:Arrb2-/- mouse 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Tumor from ApcD14/+:Arrb2-/- mouse 3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
mouse: 3
genotype/variation: ApcD14/+:Arrb2-/-
tissue: tumor
sample type: Test
|
| Growth protocol |
Tumors kept in RNA later at -80°C before RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted by RNable according to manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
For the preparation of the labelled Cy3- and Cy5- aRNA target, one microgram of total RNA samples were amplified and labelled using Amino Allyl Message Amp II aRNA amplification kit (Ambion, Austin, TX, USA), according the manufacturer’s instructions.
|
| |
|
| Channel 2 |
| Source name |
Pool of equimolar RNA of each sample
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
sample type: Reference
|
| Growth protocol |
Tumors kept in RNA later at -80°C before RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted by RNable according to manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
For the preparation of the labelled Cy3- and Cy5- aRNA target, one microgram of total RNA samples were amplified and labelled using Amino Allyl Message Amp II aRNA amplification kit (Ambion, Austin, TX, USA), according the manufacturer’s instructions.
|
| |
|
| |
| Hybridization protocol |
The two labelled aRNA were mixed, fragmented and hybridized on one array of a Nimblegen 12-plex microarray according the manufacturer's instructions.
|
| Scan protocol |
Scanned on an Innopsys Innoscan900 scanner.
|
| Description |
ApcD14-KO-3
|
| Data processing |
Images were quantified using Nimblescan V2.5. The rawdata were normalized by Lowess and a quantile normalization was applied (Limma Package).
|
| |
|
| Submission date |
Oct 07, 2010 |
| Last update date |
Jan 20, 2012 |
| Contact name |
Caroline Bonnans |
| E-mail(s) |
cbonnans@univ-montp1.fr
|
| Phone |
+33-4-67-66-81-60
|
| Organization name |
Functional Genomic Institute
|
| Department |
Oncology
|
| Street address |
141 rue de la Cardonille
|
| City |
Montpellier |
| ZIP/Postal code |
34094 |
| Country |
France |
| |
|
| Platform ID |
GPL10994 |
| Series (2) |
| GSE24575 |
beta-arrestin-2 deficiency effect on intestinal tumorigenesis in Apc-mutated mice |
| GSE24577 |
Impact of beta-arrestin-2 deficiency on intestinal tumorigenesis in Apc-mutated mice |
|
