|
| Status |
Public on Apr 16, 2023 |
| Title |
murineCB_Control_repl2 |
| Sample type |
SRA |
| |
|
| Source name |
Centroblast
|
| Organism |
Mus musculus |
| Characteristics |
sorting: B220+FAS+GL7+CXCR4+CD86-
genotype: Cgamma1Cre: +/-; Myd88L252P: WT
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA-seq libraries were prepared using the Illumina TruSeq stranded mRNA sample kits, respectively, according to the manufacturer. Libraries were validated using the Agilent Technologies 2100 Bioanalyzer and Quant-iT™ dsDNA HS Assay (Life Technologies)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
RNA-seq reads were aligned to mm10 using STAR with default parameters
TPM were obtained using Rsubread and RefSeq gene annotation
Assembly: mm10
Supplementary files format and content: tab-delimited
|
| |
|
| Submission date |
Apr 19, 2022 |
| Last update date |
Apr 17, 2023 |
| Contact name |
Matt Teater |
| E-mail(s) |
mrt2001@med.cornell.edu
|
| Organization name |
Weill Cornell Medical College
|
| Street address |
445 E 69th St
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE201058 |
Transcriptional profiling of Myd88 L252P germinal center B-cells |
|
| Relations |
| BioSample |
SAMN27642007 |
| SRA |
SRX14918034 |
