|
| Status |
Public on Sep 25, 2010 |
| Title |
liver; Bromobenzene; 75 mg/kg; 6 hours; 210556289_30633 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: left lateral liver lobe
strain: F344/N
gender: male
age: 10-12 weeks
compound: Bromobenzene
hybdate: 1/27/2005
route: Gavage
vehicle: Corn Oil
control: N
dose: 75 mg/kg
time of exposure: 6 hours
necrosis score: 2
necrosis class: 1
necrosis level (linkon): 1
necrosis level (ekl): 1
institution: NIEHS/Cogenics
|
| Treatment protocol |
Rats were exposed for 6, 24 or 48 hours to a low, medium or high dose of one of eight hepatotoxicants. All animals were fasted for 12-18 hours prior to exposure.
|
| Growth protocol |
F344/N male rats grow to 10-12 weeks old before exposed to one of eight hepatotoxicants
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the flash frozen portion of the left lateral liver lobe using RNeasy Midi columns (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
1 μg of total RNA from the left liver lobe of each individual rat was labeled using the GeneChip One-Cycle Target Labeling kit (Affymetrix, Inc., Santa Clara, CA, USA) following the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
The biotin-labeled cRNA was fragmented and hybridized to the Rat Genome 230 2.0 Array (Affymetrix, Inc.) for 17 hours, prior to washing and scanning.
|
| Scan protocol |
Data were extracted from scanned images using the Affymetrix Scanner and GeneChip Operating Software (Affymetrix, Inc.).
|
| Description |
Liver toxicants vs. non-toxicants based on overall necrosis score
MAQC_Distribution_Status: MAQC_V -- Validation
|
| Data processing |
Affymetrix MAS5
|
| |
|
| Submission date |
Sep 24, 2010 |
| Last update date |
Sep 24, 2010 |
| Contact name |
Leming Shi |
| E-mail(s) |
lemingshi@fudan.edu.cn
|
| Phone |
+86-18616827008
|
| Organization name |
Fudan University
|
| Department |
School of Life Sciences
|
| Lab |
Center for Pharmacogenomics
|
| Street address |
2005 Songhu Road
|
| City |
Shanghai |
| ZIP/Postal code |
200438 |
| Country |
China |
| |
|
| Platform ID |
GPL1355 |
| Series (2) |
| GSE16716 |
MicroArray Quality Control Phase II (MAQC-II) Project |
| GSE24363 |
MAQC-II Project: NIEHS data set |
|
