|
| Status |
Public on Dec 29, 2023 |
| Title |
M-Wnt tumor excised from C57BL6J mouse_shPC3 |
| Sample type |
RNA |
| |
|
| Source name |
M-Wnt tumor
|
| Organism |
Mus musculus |
| Characteristics |
group: shPC
strain: C57BL6J
|
| Treatment protocol |
Mice were provided 150µg/ml doxycycline in drinking water or standard drinking water ad lib
|
| Growth protocol |
50,000 M-Wnt cells transduced with PC targeting doxycycline inducible shRNA were injected into the 4th mammary fat pad of C57BL6J mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by bead beating in TRIzol reagent, followed by isolation with EZNA HP Total RNA kit (omega bio-tek)
|
| Label |
biotin
|
| Label protocol |
The GeneChip® WT PLUS Reagent Kit (Affymetrix, Santa Clara, CA) was used to prepare the samples
|
| |
|
| Hybridization protocol |
Labeled cDNA was hybridized to the plate using GeneTitan Hybridization Wash and Stain Kit for WT Arrays (Affymetrix)
|
| Scan protocol |
arrays were scanned using GeneTitan
|
| Data processing |
Quality control and differential gene expression was conducted using TAC4 software (Affymetrix)
|
| |
|
| Submission date |
Mar 30, 2022 |
| Last update date |
Dec 29, 2023 |
| Contact name |
michael francis coleman |
| E-mail(s) |
mcoleman@unc.edu
|
| Phone |
9199850347
|
| Organization name |
UNC
|
| Street address |
235 dauer drive
|
| City |
chapel hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27516 |
| Country |
USA |
| |
|
| Platform ID |
GPL24242 |
| Series (2) |
| GSE199828 |
suppression of Pyruvate carboxylase in M-wnt tumors |
| GSE199831 |
suppression of Pyruvate carboxylase |
|
