|
| Status |
Public on Jan 31, 2023 |
| Title |
Control 1 [Ctrl_2_S1] |
| Sample type |
SRA |
| |
|
| Source name |
incisor
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: wild type
tissue: incisor
age: 1month
|
| Treatment protocol |
Not applicable
|
| Growth protocol |
Mice were housed in pathogen-free conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Incisor samples from Fgfr1fl/fl (control) and Gli1-Cre;Fgfr1fl/fl (mutant) mice were collected 1 week after TMX for RNA isolation with RNeasy Micro Kit (Qiagen) according to the manufacturer’s protocol
cDNA library preparation and sequencing were performed at the Technology Center for Genomics & Bioinformatics at the University of California, Los Angeles (UCLA).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Raw reads were trimmed and aligned using TopHat (Version 2.0.8) with the mm10 genome
RPKM was used to normalize the data and differential expression was calculated by selecting transcripts that changed with p < 0.05
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include lognormal with shrinkage mean value for each feature in individual sample.
|
| |
|
| Submission date |
Mar 02, 2022 |
| Last update date |
Jan 31, 2023 |
| Contact name |
Fei Pei |
| E-mail(s) |
fp_191@usc.edu
|
| Phone |
2132454762
|
| Organization name |
USC
|
| Street address |
2250 Alcazar St
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL21103 |
| Series (2) |
| GSE197785 |
Sensory nerve niche regulates mesenchymal stem cell homeostasis via FGF/mTOR/autophagy axis [RNA-seq] |
| GSE197787 |
Sensory nerve niche regulates mesenchymal stem cell homeostasis via FGF/mTOR/autophagy axis. |
|
| Relations |
| BioSample |
SAMN26366568 |
| SRA |
SRX14353525 |
