|
| Status |
Public on Sep 18, 2012 |
| Title |
SKNO-1_AE (A706_Diagenode)_no |
| Sample type |
SRA |
| |
|
| Source name |
SKNO-1 (leukemic)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: hematopoietic cell
disease state: leukemic
treatment: no
antibody: AE (A706)
antibody vendor: n/a (commercialization pending manuscript publication)
antibody catalog number: n/a (commercialization pending manuscript publication)
antibody lot/batch number: n/a (commercialization pending manuscript publication)
|
| Treatment protocol |
U937AML1ETO cells were treated with zinc for 5 hours.
|
| Growth protocol |
Leukemic cells were cultured in normal cell culture containing 10% serum.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin was fixed in 1% formaldehyde for 10 minutes, sheared to 200-700bp using a Bioruptor (Diagenode), immunoprecipitated using the indicated antisera, purified and sequenced using Illumina Genome Analyzers as recommended by the manufacturer.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
chromatin IP against AE (A706)
|
| Data processing |
Reads (32 bp) were aligned to the Human (Homo Sapiens) reference genome HG18. Peaks were called using MACS. MethylCap tracks for the different fractions were equalized for the total number of mapped tags by random removal of sequenced tags and combined.
|
| |
|
| Submission date |
Aug 20, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
H.G. Stunnenberg |
| E-mail(s) |
h.stunnenberg@ncmls.ru.nl
|
| Phone |
+31-24-3610520
|
| Organization name |
Radboud University
|
| Department |
Department of Molecular Biology (274)
|
| Street address |
Geert Grooteplein 28
|
| City |
Nijmegen |
| ZIP/Postal code |
6525 GA |
| Country |
USA |
| |
|
| Platform ID |
GPL9052 |
| Series (1) |
| GSE23730 |
Oncofusion protein AML1-ETO targets ERG factor binding sites in AML |
|
| Relations |
| SRA |
SRX026638 |
| BioSample |
SAMN00113179 |
