|
| Status |
Public on Feb 15, 2022 |
| Title |
B10-2D2_S66 SCC Bulk RNAseq SCC101 |
| Sample type |
SRA |
| |
|
| Source name |
Peripheral Blood Treg Cells (SCC)
|
| Organism |
Homo sapiens |
| Characteristics |
phenotype: CD45+ CD3+ CD4+ CD24+ CD127- IL1R1-
donors: SCC101
stimulation: anti-CD3/CD28/CD2 beads and IL-1b
timepoint: Day 2
tissue: Peripheral Blood
|
| Treatment protocol |
Tregs were sorted and cultured with either no stimulation, stimulation with anti-CD3/CD28/CD2 beads, or stimulation with anti-CD3/CD28/CD2 beads and IL-1b for 1, 2, or 3 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using SMART-Seq v4 Ultra Low Input RNA kit protocols for bulk RNA-seq
RNA was constructed using NexteraXT DNA sample preperation protocol for bulk RNA-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Description |
B10-2D2_S66
|
| Data processing |
Bulk RNAseq samples were processed using Galaxy, alighed with STARaligner
Genome_build: GRCh38
Supplementary_files_format_and_content: comma separated values with abundance measurements for each target.
|
| |
|
| Submission date |
Jan 26, 2022 |
| Last update date |
Feb 15, 2022 |
| Contact name |
Martin Prlic |
| E-mail(s) |
mprlic@fredhutch.org
|
| Phone |
2066672216
|
| Organization name |
Fred Hutch
|
| Street address |
1100 Fairview Ave, Mail stop E5-110
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98108 |
| Country |
USA |
| |
|
| Platform ID |
GPL30173 |
| Series (1) |
| GSE163633 |
Identification of human tumor-unique signaling networks between antigen-presenting cells and T cells using multi-omic single cell analysis |
|
| Relations |
| BioSample |
SAMN25287807 |
| SRA |
SRX13935421 |
