|
| Status |
Public on Aug 14, 2010 |
| Title |
ovarian epithelial cells T80_without HMGA2_rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
immortalized ovarian surface epithelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: immortalized ovarian epithelial cell line T80
transfection: control
|
| Growth protocol |
Cells were maintained at 37oC in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by miRNA isolation kit from Ambion according to manufacturer’s protocol.
|
| Label |
Cy5
|
| Label protocol |
The labeling was accomplished by Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion) according to manufacturer’s protocol. Firstly, cDNA was synthesized by reverse transcription, including first and second strand synthesis. Then the purified cDNA became the template for in vitro transcription with T7 RNA polymerase. In this step, the modified nucleotide, 5-(3-aminoallyl)-UTP (aaUTP) will be incorporated into the aRNA which would be subsequently labeled by Cy5 dye.
|
| |
|
| Hybridization protocol |
The array was hybridized by using OneArray Hybridization Buffer (Phalanx) for 14 to 16h at 420C which was followed by washing two times per manufacturer’s protocol.
|
| Scan protocol |
The chips were scanned by Axon 4000 scanner (Molecular Devices).
|
| Description |
None
|
| Data processing |
Hierarchical clustering the expression profiles was done by software Cluster 3.0. The genes which have different expression with fold>2 were further analyzed by Pathway-Express.
|
| |
|
| Submission date |
Aug 12, 2010 |
| Last update date |
Aug 13, 2010 |
| Contact name |
Jingjing Wu |
| E-mail(s) |
wu_jingjing2007@yahoo.com.cn
|
| Phone |
847-530-3863
|
| Organization name |
northwestern university
|
| Department |
pathology
|
| Lab |
ward building 3-031
|
| Street address |
303 E. Chicago Avenue
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL6254 |
| Series (1) |
| GSE23599 |
The gene profile analysis of the function of HMGA2 in immortalized human ovarian surface epithelial cell lines. |
|
